Find your answer

We share answers to frequently asked questions about sample preparation for cryo-EM

What grids can we use at the VitroJet?

You can use any pre-clipped autogrid. This includes standard grids from different manufacturers, as well as custom-made grids, and grids with continuous films.

How can we modify settings for plasma treatment with the VitroJet?

The plasma treatment of grids is integrated in the VitroJet to ensure reproducibility between grids. The main parameter that is tuned is duration depending on the type of grid that is introduced in the VitroJet. However, if you prefer to treat your grids in a specific way, the plasma treatment step inside the VitroJet can be bypassed.

How many grids can we produce in a single session with the VitroJet?

The VitroJet cryo-EM sample preparation solution is designed for you to process up to 12 pre-clipped autogrids per session.

How long does the nitrogen/ethane last with the VitroJet?

The VitroJet is equipped with a system that automatically refills both liquid nitrogen and ethane. The degree of contamination determines how long you can work with the VitroJet. It is recommended to use the VitroJet in a low-humidity environment.

Are we restricted to a type or brand of microscope when we prepare samples with the VitroJet?

No, you are not restricted to a type or brand of microscope when you prepare samples with the VitroJet. The VitroJet is designed for automation and control. Therefor the VitroJet is very flexible, allowing any pre-clipped autogrid to be prepared and subsequently handled in any microscope. Even side-entry cryo-holders can be adjusted with only minor changes to fit pre-clipped grids. Please reach out to us in case you have remaining questions about compatibility with your microscope or cryo-holder.

Are we restricted to only one sample per session with the VitroJet?

No, you are not restricted to one sample per session with the VitroJet. You can change your sample at any point in the session. This applies to any other parameter you wish to adjust in the session.

Where can we place the VitroJet?

The VitroJet is a stand-alone device. It has a footprint of W x D x H = 70 x 85 x 170cm / 27 x 31 x 67 inch. Additionally, you require a bench space for a pin drum cleaning station and the instrument control PC, including two monitors, a keyboard and a mouse. Although the VitroJet is a closed system, ideally the environment where you place the VitroJet has a relative low humidity and limited flow, to ensure minimal ice contamination. The ambient operating conditions include a temperature of 17-22 °C and a relative humidity below 40%.

What are the requirements for connections to the VitroJet?

The VitroJet is designed to operate at 230 VAC/50 Hz and/or 110 VAC/60 Hz. You require a wall connection with dry nitrogen gas is required. Gas cylinders with ethane and artificial air are connected. Distilled water is used for obtaining the humidity in the sample deposition module.